NEIST-SOPHISTICATED ANALYTICAL INSTRUMENT FACILITY (NEIST-SAIF)

Sophisticated Analytical Instruments Facility

Name of the equipment/facility: Fluorescence Activated Cell Sorter (FACS) & Flow Cytometry
Make: Beckman Coulter, USA
Model:MoFlo XDP&CytoFLEX S
Specification:
Lasers - Lasers have fixed optical bench and lasers with wavelength ranges from minimum of 488nm to 633nm with 3 lasers such as 488nm Blue laser; 561 YG laser and 633 Red laser. System is capable of simultaneous operation of all the quoted lasers. The system has provision for future upgradability with more lasers.
Parameters: - Provided with Forward Scatter, Side Scatter & simultaneous 09 or more colors/ fluorescence detectors. - Has got fixed align/ minimum align optical arrays of octagon and trigons, Jet in Air technology - Analysis speeds of up to 100,000 events / second - Exhibits sorting speeds of up to 70,000 events / second - Fluidics cart includes sheath, waste, and cleaning containers - ACDU module provided with automated sorting into 6, 24, 48, 96, and 384-well plates and slides - Contain sample injection chamber for various sample input tubes, including microtubes, 12 x 75-mm, and 15 ml tubes - Nozzle size in the range of 70, 85, 100, and 130-micron sizes - Purity: 98% post sorting
Applications:
Flow cytometry is considered to be a promising high-throughput technique for its ability to interrogate living cells at the single-cell level. Flow cytometry has become an extremely valuable tool in the microbiology, molecular biology, metabolic engineering, protein engineering and drug development research. As applications, in biology laboratory, flow cytometry enables the analysis of the different features or physiological states of bacteria, plant, fungi, microalgae, yeast and mammalian cells at the single cell level. In drug development laboratories, flow cytometry helps for identification of high content analysis, pre-clinincal drug development, imaging cytometry, phosphor-specific drug screening, and receptor pharmacology.By using flow cytometry, we planned to divide the heterogeneous microalgal population into different parts with respect to their lipid content and isolate the high, medium and low-lipid content cells of haptophycean microalgae at single-cell level; to develop novel techniques for microbial strain development. In details, flow cytometry will be helpful to enrich the particular number of cells from a heterogeneous population for specific applications
User Instructions:
- User has to bring their samples personally or send through proper shipping under controlled conditions - User has to provide sample details about the type of samples and purpose, whether to perform sorting, analysis or both. - If sorting is required, user should bring their own test tubes or 96 well plates. - Users are encouraged to be present during the analysis of their samples. Users who are interested in specialised experiments are advised to contact Central Instrumentation facility, Bhatnagar Building of CSIR-NEIST, Jorhat-785 006 for an appointment. - All samples should accompany material safety data sheets (MSDS).
Contact
Dr.ChannaChikkaputtaiah, Senior Scientist, CSIR-NEIST Jorhat-785006. E-mail:channakeshav@neist.res.in
Charges (Excluding Taxes)

Particulars

Industry

University

National Labs

Per sample/per hour

Analyzer

 

 

 

 

Instrumentation
Operator assisted
Expertise assisted

2200
2650
3050

1750
2100
2410

1750
2100
2410

Per hour
Per hour
Per hour

Sorter

 

 

 

 

Instrumentation
Operator assisted
Expertise assisted

4540
4950
5375

3600
3950
4260

3600
3950
4260

Per hour
Per hour
Per hour